Fluorescent indicators for simultaneous reporting of all four cell cycle phases.
basic_science · Level V
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- Record sourced from PubMed, PMID 27798610.
- Also identified by DOI 10.1038/nmeth.4045 and PMC identifier 5548384.
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Abstract
A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.
Medical subject headings
- Cell Cycle
- Luminescent Proteins
- Molecular Imaging
- Recombinant Fusion Proteins
- Time-Lapse Imaging