Saponin-based adjuvants induce cross-presentation in dendritic cells by intracellular lipid body formation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27819292.
- Also identified by DOI 10.1038/ncomms13324 and PMC identifier 5103066.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Saponin-based adjuvants (SBAs) are being used in animal and human (cancer) vaccines, as they induce protective cellular immunity. Their adjuvant potency is a factor of inflammasome activation and enhanced antigen cross-presentation by dendritic cells (DCs), but how antigen cross-presentation is induced is not clear. Here we show that SBAs uniquely induce intracellular lipid bodies (LBs) in the CD11b+ DC subset in vitro and in vivo. Using genetic and pharmacological interference in models for vaccination and in situ tumour ablation, we demonstrate that LB induction is causally related to the saponin-dependent increase in cross-presentation and T-cell activation. These findings link adjuvant activity to LB formation, aid the application of SBAs as a cancer vaccine component, and will stimulate development of new adjuvants enhancing T-cell-mediated immunity.
Medical subject headings
- Cancer Vaccines
- Cross-Priming
- Dendritic Cells
- Melanoma, Experimental
- Saponins
- Skin Neoplasms