Mass and stiffness spectrometry of nanoparticles and whole intact bacteria by multimode nanomechanical resonators.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27834379.
- Also identified by DOI 10.1038/ncomms13452 and PMC identifier 5476793.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The identification of species is a fundamental problem in analytical chemistry and biology. Mass spectrometers identify species by their molecular mass with extremely high sensitivity (<10<sup>-24</sup> g). However, its application is usually limited to light analytes (<10<sup>-19</sup> g). Here we demonstrate that by using nanomechanical resonators, heavier analytes can be identified by their mass and stiffness. The method is demonstrated with spherical gold nanoparticles and whole intact E. coli bacteria delivered by electrospray ionization to microcantilever resonators placed in low vacuum at 0.1 torr. We develop a theoretical procedure for obtaining the mass, position and stiffness of the analytes arriving the resonator from the adsorption-induced eigenfrequency jumps. These results demonstrate the enormous potential of this technology for identification of large biological complexes near their native conformation, a goal that is beyond the capabilities of conventional mass spectrometers.
Medical subject headings
- Escherichia coli
- Gold
- Mechanotransduction, Cellular
- Metal Nanoparticles
- Nanotechnology
- Spectrum Analysis