Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27848933.
- Also identified by DOI 10.1038/ncomms13274 and PMC identifier 5116081.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR-Cas9 has quickly become the technology of choice for genome editing due to its simplicity, efficiency and versatility. Currently, genome editing in plants primarily relies on delivering double-strand break reagents in the form of DNA vectors. Here we report biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles. Using this method of delivery, we also demonstrate DNA- and selectable marker-free gene mutagenesis in maize and recovery of plants with mutated alleles at high frequencies. These results open new opportunities to accelerate breeding practices in a wide variety of crop species.
Medical subject headings
- CRISPR-Cas Systems
- Gene Editing
- Multiprotein Complexes
- Ribonucleoproteins
- Zea mays