CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes.
review · Level V
Where this comes from
- Record sourced from PubMed, PMID 27866654.
- Also identified by DOI 10.1016/j.cell.2016.10.044 and PMC identifier 5235943.
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Abstract
The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. In this Review, we summarize CRISPR-based technologies that enable mammalian genome editing and their various applications. We describe recent developments that extend the generality, DNA specificity, product selectivity, and fundamental capabilities of natural CRISPR systems, and we highlight some of the remarkable advancements in basic research, biotechnology, and therapeutics science that these developments have facilitated.
Medical subject headings
- Gene Editing