Covalently circularized nanodiscs for studying membrane proteins and viral entry.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27869813.
- Also identified by DOI 10.1038/nmeth.4079 and PMC identifier 5199620.
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Abstract
We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter sizes and tunable shapes. Reconstitution into cNDs enhanced the quality of nuclear magnetic resonance spectra for both VDAC-1, a β-barrel membrane protein, and the G-protein-coupled receptor NTR1, an α-helical membrane protein. In addition, we used cNDs to visualize how simple, nonenveloped viruses translocate their genomes across membranes to initiate infection.
Medical subject headings
- Lipid Bilayers
- Nanostructures
- Receptors, Neurotensin
- Voltage-Dependent Anion Channel 1