mScarlet: a bright monomeric red fluorescent protein for cellular imaging.
basic_science · Level V
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- Record sourced from PubMed, PMID 27869816.
- Also identified by DOI 10.1038/nmeth.4074.
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Abstract
We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.
Medical subject headings
- Bacteria
- Bacterial Proteins
- Fluorescence Resonance Energy Transfer
- Luminescent Proteins
- Molecular Imaging
- Protein Engineering