Modular delivery of CpG-incorporated lipid-DNA nanoparticles for spleen DC activation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27886556.
- Also identified by DOI 10.1016/j.biomaterials.2016.11.020.
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Abstract
We introduce a versatile carrier system for in vitro and in vivo immune stimulation based on soft matter DNA nanoparticles (NPs). The incorporation of lipid-modified nucleotides into DNA strands enables the formation of micelles of uniform size. In a single self-assembly step, the micelles can be equipped with immune adjuvant (CpG) motifs and fluorescent probes. The immunological effects of CpG confined at the NP surface were studied in a comprehensive manner in animal experiments. Dose-dependent activation of spleen dendritic cells (DCs) by CpG-conjugated NP was observed, which was accompanied by the pronounced up-regulation of co-stimulatory molecule and cytokine production.
Medical subject headings
- CpG Islands
- DNA
- Dendritic Cells
- Lipids
- Nanocapsules
- Spleen