Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27918548.
- Also identified by DOI 10.1038/nbt.3737 and PMC identifier 5225075.
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Abstract
Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.
Medical subject headings
- Bacterial Proteins
- CRISPR-Associated Proteins
- CRISPR-Cas Systems
- Gene Editing
- RNA, Bacterial
- Sequence Analysis, RNA