A High-Throughput Strategy for Dissecting Mammalian Genetic Interactions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27936040.
- Also identified by DOI 10.1371/journal.pone.0167617 and PMC identifier 5147906.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Comprehensive delineation of complex cellular networks requires high-throughput interrogation of genetic interactions. To address this challenge, we describe the development of a multiplex combinatorial strategy to assess pairwise genetic interactions using CRISPR-Cas9 genome editing and next-generation sequencing. We characterize the performance of combinatorial genome editing and analysis using different promoter and gRNA designs and identified regions of the chimeric RNA that are compatible with next-generation sequencing preparation and quantification. This approach is an important step towards elucidating genetic networks relevant to human diseases and the development of more efficient Cas9-based therapeutics.
Medical subject headings
- CRISPR-Cas Systems
- Gene Editing
- Gene Regulatory Networks
- RNA, Guide, CRISPR-Cas Systems