Deletion of the sclerotome-enriched lncRNA PEAT augments ribosomal protein expression.
basic_science · Level V
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- Record sourced from PubMed, PMID 27986952.
- Also identified by DOI 10.1073/pnas.1612069113 and PMC identifier 5224379.
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Abstract
To define a complete catalog of the genes that are activated during mouse sclerotome formation, we sequenced RNA from embryonic mouse tissue directed to form sclerotome in culture. In addition to well-known early markers of sclerotome, such as Pax1, Pax9, and the Bapx2/Nkx3-2 homolog Nkx3-1, the long-noncoding RNA PEAT (Pax1 enhancer antisense transcript) was induced in sclerotome-directed samples. Strikingly, PEAT is located just upstream of the Pax1 gene. Using CRISPR/Cas9, we generated a mouse line bearing a complete deletion of the PEAT-transcribed unit. RNA-seq on PEAT mutant embryos showed that loss of PEAT modestly increases bone morphogenetic protein target gene expression and also elevates the expression of a large subset of ribosomal protein mRNAs.
Medical subject headings
- Embryonic Development
- Gene Expression Regulation, Developmental
- Paired Box Transcription Factors
- RNA, Long Noncoding
- RNA, Ribosomal
- Ribosomal Proteins