cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans.
basic_science · Level V
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- Record sourced from PubMed, PMID 27992408.
- Also identified by DOI 10.1038/nmeth.4109 and PMC identifier 5693259.
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Abstract
The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit.
Medical subject headings
- Caenorhabditis elegans
- DNA-Binding Proteins
- Gene Expression Regulation
- Genetic Engineering
- Saccharomyces cerevisiae Proteins
- Transcription Factors