Light-Enhancing Plasmonic-Nanopore Biosensor for Superior Single-Molecule Detection.

Assad, Ossama N; Gilboa, Tal; Spitzberg, Joshua; Juhasz, Matyas; Weinhold, Elmar; Meller, Amit · Adv Mater · 2017

basic_science · Level V

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Abstract

A stacked plasmonic nanowell-nanopore biosensor strongly suppresses the background fluorescence from the bulk and yields net more than tenfold enhancement of the fluorescence intensity. The device offers extremely high signal-to-background (S/B) ratio for single-molecule detection at ultralow excitation laser intensities, while maintaining extremely high temporal bandwidth for single-DNA sensing.

Medical subject headings