Thin-Film Transistor-Based Biosensors for Determining Stoichiometry of Biochemical Reactions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28033412.
- Also identified by DOI 10.1371/journal.pone.0169094 and PMC identifier 5199051.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The enzyme kinetic in a biochemical reaction is critical to scientific research and drug discovery but can hardly be determined experimentally from enzyme assays. In this work, a charge-current transducer (a transistor) is proposed to evaluate the status of biochemical reaction by monitoring the electrical charge changes. Using the malate-aspartate shuttle as an example, a thin-film transistor (TFT)-based biosensor with an extended gold pad is demonstrated to detect the biochemical reaction between NADH and NAD+. The drain current change indicates the status of chemical equilibrium and stoichiometry.
Medical subject headings
- Biosensing Techniques
- Transistors, Electronic