Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28068315.
- Also identified by DOI 10.1038/nmeth.4134.
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Abstract
Time-lapse imaging of multiple labels is challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quality, while throughput can be limited by processing time. Here, we report software called Hyper-Spectral Phasors (HySP) for denoising and unmixing multiple spectrally overlapping fluorophores in a low signal-to-noise regime with fast analysis. We show that HySP enables unmixing of seven signals in time-lapse imaging of living zebrafish embryos.
Medical subject headings
- Software
- Time-Lapse Imaging