Cryogenic optical localization provides 3D protein structure data with Angstrom resolution.
basic_science · Level V
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- Record sourced from PubMed, PMID 28068317.
- Also identified by DOI 10.1038/nmeth.4141.
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Abstract
We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize multiple fluorescent sites within a single small protein with Angstrom resolution. We demonstrate COLD by determining the conformational state of the cytosolic Per-ARNT-Sim domain from the histidine kinase CitA of Geobacillus thermodenitrificans and resolving the four biotin sites of streptavidin. COLD provides quantitative 3D information about small- to medium-sized biomolecules on the Angstrom scale and complements other techniques in structural biology.
Medical subject headings
- Fluorescent Dyes
- Histidine Kinase
- Microscopy, Fluorescence
- Optics and Photonics
- Single Molecule Imaging