Adipose Tissue CLK2 Promotes Energy Expenditure during High-Fat Diet Intermittent Fasting.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28089567.
- Also identified by DOI 10.1016/j.cmet.2016.12.007 and PMC identifier 5299049.
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Abstract
A promising approach to treating obesity is to increase diet-induced thermogenesis in brown adipose tissue (BAT), but the regulation of this process remains unclear. Here we find that CDC-like kinase 2 (CLK2) is expressed in BAT and upregulated upon refeeding. Mice lacking CLK2 in adipose tissue exhibit exacerbated obesity and decreased energy expenditure during high-fat diet intermittent fasting. Additionally, tissue oxygen consumption and protein levels of UCP1 are reduced in CLK2-deficient BAT. Phosphorylation of CREB, a transcriptional activator of UCP1, is markedly decreased in BAT cells lacking CLK2 due to enhanced CREB dephosphorylation. Mechanistically, CREB dephosphorylation is rescued by the inhibition of PP2A, a phosphatase that targets CREB. Our results suggest that CLK2 is a regulatory component of diet-induced thermogenesis in BAT through increased CREB-dependent expression of UCP1.
Medical subject headings
- Adipose Tissue
- Diet, High-Fat
- Energy Metabolism
- Fasting
- Protein Serine-Threonine Kinases
- Protein-Tyrosine Kinases