Post-translational selective intracellular silencing of acetylated proteins with de novo selected intrabodies.
basic_science · Level V
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- Record sourced from PubMed, PMID 28092690.
- Also identified by DOI 10.1038/nmeth.4144.
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Abstract
The ability to selectively interfere with post-translationally modified proteins would have many biological and therapeutic applications. However, post-translational modifications cannot be selectively targeted by nucleic-acid-based interference approaches. Here we describe post-translational intracellular silencing antibody technology (PISA), a method for selecting intrabodies against post-translationally modified proteins. We demonstrate our method by generating intrabodies against native acetylated proteins and showing functional interference in living cells.
Medical subject headings
- Antibodies
- HIV Integrase
- Histones
- Protein Processing, Post-Translational