The RFamide receptor DMSR-1 regulates stress-induced sleep in <i>C. elegans</i>.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28094002.
- Also identified by DOI 10.7554/eLife.19837 and PMC identifier 5241116.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
In response to environments that cause cellular stress, animals engage in sleep behavior that facilitates recovery from the stress. In <i>Caenorhabditis elegans</i>, stress-induced sleep(SIS) is regulated by cytokine activation of the ALA neuron, which releases FLP-13 neuropeptides characterized by an amidated arginine-phenylalanine (RFamide) C-terminus motif. By performing an unbiased genetic screen for mutants that impair the somnogenic effects of FLP-13 neuropeptides, we identified the gene <i>dmsr-1</i>, which encodes a G-protein coupled receptor similar to an insect RFamide receptor. DMSR-1 is activated by FLP-13 peptides in cell culture, is required for SIS <i>in vivo</i>, is expressed non-synaptically in several wake-promoting neurons, and likely couples to a Gi/o heterotrimeric G-protein. Our data expand our understanding of how a single neuroendocrine cell coordinates an organism-wide behavioral response, and suggest that similar signaling principles may function in other organisms to regulate sleep during sickness.
Medical subject headings
- Caenorhabditis elegans
- Caenorhabditis elegans Proteins
- Gene Expression Regulation
- Neuropeptides
- Receptors, Peptide
- Sleep
- Stress, Physiological