Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 28094784.
- Also identified by DOI 10.1038/ncomms13913 and PMC identifier 5247573.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Molecular diagnostics is typically outsourced to well-equipped centralized laboratories, often far from the patient. We developed molecular assays and portable optical imaging designs that permit on-site diagnostics with a cost-effective mobile-phone-based multimodal microscope. We demonstrate that targeted next-generation DNA sequencing reactions and in situ point mutation detection assays in preserved tumour samples can be imaged and analysed using mobile phone microscopy, achieving a new milestone for tele-medicine technologies.
Medical subject headings
- Cell Phone
- DNA
- Neoplasms
- Pathology, Molecular
- Sequence Analysis, DNA