High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28114288.
- Also identified by DOI 10.1038/nmeth.4147.
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Abstract
Investigation of the structure, assembly and function of protein-nucleic acid macromolecular machines requires multidimensional molecular and structural biology approaches. We describe modifications to an Orbitrap mass spectrometer, enabling high-resolution native MS analysis of 0.8- to 2.3-MDa prokaryotic 30S, 50S and 70S ribosome particles and the 9-MDa Flock House virus. The instrument's improved mass range and sensitivity readily exposes unexpected binding of the ribosome-associated protein SRA.
Medical subject headings
- Escherichia coli
- Mass Spectrometry
- Nodaviridae
- RNA, Long Noncoding
- Ribosome Subunits, Large, Bacterial
- Ribosome Subunits, Small, Bacterial