Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28129503.
- Also identified by DOI 10.1021/acsnano.6b07600 and PMC identifier 5848212.
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Abstract
Genome editing through the delivery of CRISPR/Cas9-ribonucleoprotein (Cas9-RNP) reduces unwanted gene targeting and avoids integrational mutagenesis that can occur through gene delivery strategies. Direct and efficient delivery of Cas9-RNP into the cytosol followed by translocation to the nucleus remains a challenge. Here, we report a remarkably highly efficient (∼90%) direct cytoplasmic/nuclear delivery of Cas9 protein complexed with a guide RNA (sgRNA) through the coengineering of Cas9 protein and carrier nanoparticles. This construct provides effective (∼30%) gene editing efficiency and opens up opportunities in studying genome dynamics.
Medical subject headings
- CRISPR-Cas Systems
- Cytosol
- Gene Editing
- Gene Transfer Techniques
- Ribonucleoproteins