H(O)TA: estimation of DNA methylation and hydroxylation levels and efficiencies from time course data.
basic_science · Level V
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- Record sourced from PubMed, PMID 28130236.
- Also identified by DOI 10.1093/bioinformatics/btx042.
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Abstract
Methylation and hydroxylation of cytosines to form 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) belong to the most important epigenetic modifications and their vital role in the regulation of gene expression has been widely recognized. Recent experimental techniques allow to infer methylation and hydroxylation levels at CpG dinucleotides but require a sophisticated statistical analysis to achieve accurate estimates. We present H(O)TA, a software tool based on a stochastic modeling approach, which simultaneously analyzes time course data from hairpin bisulfite sequencing and hairpin oxidative bisulfite sequencing. : https://mosi.uni-saarland.de/HOTA. charalampos.kyriakopoulos@uni-saarland.de or verena.wolf@uni-saarland.de.
Medical subject headings
- CpG Islands
- DNA
- DNA Methylation
- Sequence Analysis, DNA
- Software