Massively multiplex single-cell Hi-C.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28135255.
- Also identified by DOI 10.1038/nmeth.4155 and PMC identifier 5330809.
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Abstract
We present single-cell combinatorial indexed Hi-C (sciHi-C), a method that applies combinatorial cellular indexing to chromosome conformation capture. In this proof of concept, we generate and sequence six sciHi-C libraries comprising a total of 10,696 single cells. We use sciHi-C data to separate cells by karyotypic and cell-cycle state differences and identify cell-to-cell heterogeneity in mammalian chromosomal conformation. Our results demonstrate that combinatorial indexing is a generalizable strategy for single-cell genomics.
Medical subject headings
- Chromosomes
- DNA
- Genome, Human
- Genomics
- Molecular Conformation
- Single-Cell Analysis