One-step generation of conditional and reversible gene knockouts.
basic_science · Level V
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- Record sourced from PubMed, PMID 28135257.
- Also identified by DOI 10.1038/nmeth.4156 and PMC identifier 5777571.
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Abstract
Loss-of-function studies are key for investigating gene function, and CRISPR technology has made genome editing widely accessible in model organisms and cells. However, conditional gene inactivation in diploid cells is still difficult to achieve. Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid and scalable method for biallelic conditional gene knockouts in diploid or aneuploid cells, such as pluripotent stem cells, 3D organoids and cell lines, by co-delivery of CRISPR-Cas9 and a universal conditional intronic cassette.
Medical subject headings
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Embryonic Stem Cells
- Gene Editing
- Gene Knockout Techniques
- beta Catenin