One-step micromolding of complex 3D microchambers for single-cell analysis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28150829.
- Also identified by DOI 10.1039/c6lc01313a.
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Abstract
Herein we examined the extent of replicability of the PDMS microchamber device transferred from the master mold with complex 3D structures fabricated via micro stereolithography. Due to the elastomeric properties of PDMS, the reversely tapered micromold, with the diameter ratio of ∼5 from the largest to the narrowest part, was precisely transferred without breaking. We obtained the mathematical model to estimate the stress exerted on the mold during the demolding process. Finally, we tested the applicability of this unusual microchamber for single-cell trapping and an enzyme assay.
Medical subject headings
- Cell Culture Techniques
- Microfluidic Analytical Techniques
- Single-Cell Analysis