PPR-SMR protein SOT1 has RNA endonuclease activity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28167782.
- Also identified by DOI 10.1073/pnas.1612460114 and PMC identifier 5338415.
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Abstract
Numerous attempts have been made to identify and engineer sequence-specific RNA endonucleases, as these would allow for efficient RNA manipulation. However, no natural RNA endonuclease that recognizes RNA in a sequence-specific manner has been described to date. Here, we report that SUPPRESSOR OF THYLAKOID FORMATION 1 (SOT1), an <i>Arabidopsis</i> pentatricopeptide repeat (PPR) protein with a small MutS-related (SMR) domain, has RNA endonuclease activity. We show that the SMR moiety of SOT1 performs the endonucleolytic maturation of 23S and 4.5S rRNA through the PPR domain, specifically recognizing a 13-nucleotide RNA sequence in the 5' end of the chloroplast 23S-4.5S rRNA precursor. In addition, we successfully engineered the SOT1 protein with altered PPR motifs to recognize and cleave a predicted RNA substrate. Our findings point to SOT1 as an exciting tool for RNA manipulation.
Medical subject headings
- Arabidopsis
- Arabidopsis Proteins
- Endoribonucleases
- RNA, Chloroplast
- Thylakoids