Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
basic_science · Level V
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- Record sourced from PubMed, PMID 28192420.
- Also identified by DOI 10.1038/nmeth.4178 and PMC identifier 5376236.
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Abstract
Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.
Medical subject headings
- Cryoelectron Microscopy
- Crystallography
- Proteins