Mapping DNA methylation with high-throughput nanopore sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28218897.
- Also identified by DOI 10.1038/nmeth.4189 and PMC identifier 5704956.
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Abstract
DNA chemical modifications regulate genomic function. We present a framework for mapping cytosine and adenosine methylation with the Oxford Nanopore Technologies MinION using this nanopore sequencer's ionic current signal. We map three cytosine variants and two adenine variants. The results show that our model is sensitive enough to detect changes in genomic DNA methylation levels as a function of growth phase in Escherichia coli.
Medical subject headings
- 5-Methylcytosine
- DNA Methylation
- High-Throughput Nucleotide Sequencing
- Nanopores