Detecting DNA cytosine methylation using nanopore sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28218898.
- Also identified by DOI 10.1038/nmeth.4184.
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Abstract
In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect for the Oxford Nanopore Technologies MinION sequencer. By using synthetically methylated DNA, we were able to train a hidden Markov model to distinguish 5-mC from unmethylated cytosine. We applied our method to sequence the methylome of human DNA, without requiring special steps for library preparation.
Medical subject headings
- 5-Methylcytosine
- Cytosine
- DNA Methylation
- Genome, Human