In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28218900.
- Also identified by DOI 10.1038/nmeth.4183 and PMC identifier 6441362.
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Abstract
High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at ∼1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resolution deep within scattering brain tissues.
Medical subject headings
- Brain
- Microscopy, Fluorescence, Multiphoton
- Neurons