Expanding the genetic code of Mus musculus.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28220771.
- Also identified by DOI 10.1038/ncomms14568 and PMC identifier 5321798.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including N<sup>ɛ</sup>-acetyl-lysine. Stable integration of transgenes encoding an engineered N<sup>ɛ</sup>-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNA<sup>Pyl</sup> pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.
Medical subject headings
- Amino Acids
- Amino Acyl-tRNA Synthetases
- Genetic Code
- Green Fluorescent Proteins
- Lysine
- RNA, Transfer