Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28244994.
- Also identified by DOI 10.1038/nbt.3811.
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Abstract
Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.
Medical subject headings
- CRISPR-Associated Proteins
- Clustered Regularly Interspaced Short Palindromic Repeats
- DNA, Plant
- Gene Editing
- Mutagenesis, Site-Directed
- Plants