Highly efficient RNA-guided base editing in mouse embryos.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28244995.
- Also identified by DOI 10.1038/nbt.3816.
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Abstract
Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.
Medical subject headings
- CRISPR-Associated Proteins
- Clustered Regularly Interspaced Short Palindromic Repeats
- Embryo, Mammalian
- Gene Editing
- Mutagenesis, Site-Directed
- RNA