A microRNA-initiated DNAzyme motor operating in living cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28262725.
- Also identified by DOI 10.1038/ncomms14378 and PMC identifier 5343503.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Synthetic DNA motors have great potential to mimic natural protein motors in cells but the operation of synthetic DNA motors in living cells remains challenging and has not been demonstrated. Here we report a DNAzyme motor that operates in living cells in response to a specific intracellular target. The whole motor system is constructed on a 20 nm gold nanoparticle (AuNP) decorated with hundreds of substrate strands serving as DNA tracks and dozens of DNAzyme molecules each silenced by a locking strand. Intracellular interaction of a target molecule with the motor system initiates the autonomous walking of the motor on the AuNP. An example DNAzyme motor responsive to a specific microRNA enables amplified detection of the specific microRNA in individual cancer cells. Activated by specific intracellular targets, these self-powered DNAzyme motors will have diverse applications in the control and modulation of biological functions.
Medical subject headings
- DNA, Catalytic
- Gold
- Manganese
- Metal Nanoparticles
- MicroRNAs
- Molecular Motor Proteins