GuideScan software for improved single and paired CRISPR guide RNA design.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28263296.
- Also identified by DOI 10.1038/nbt.3804 and PMC identifier 5607865.
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Abstract
We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.
Medical subject headings
- Algorithms
- Clustered Regularly Interspaced Short Palindromic Repeats
- Gene Silencing
- Machine Learning
- RNA, Small Interfering
- Software