Diagnosis of Zika virus infection on a nanotechnology platform.

Zhang, Bo; Pinsky, Benjamin A; Ananta, Jeyarama S; Zhao, Su; Arulkumar, Shylaja; Wan, Hao; Sahoo, Malaya K; Abeynayake, Janaki et al. · Nat Med · 2017

basic_science · Level V

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Abstract

We developed a multiplexed assay on a plasmonic-gold platform for measuring IgG and IgA antibodies and IgG avidity against both Zika virus (ZIKV) and dengue virus (DENV) infections. In contrast to IgM cross-reactivity, IgG and IgA antibodies against ZIKV nonstructural protein 1 (NS1) antigen were specific to ZIKV infection, and IgG avidity revealed recent ZIKV infection and past DENV-2 infection in patients in dengue-endemic regions. This assay could enable specific diagnosis of ZIKV infection over other flaviviral infections.

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