Optogenetic control with a photocleavable protein, PhoCl.
basic_science · Level V
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- Record sourced from PubMed, PMID 28288123.
- Also identified by DOI 10.1038/nmeth.4222.
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Abstract
To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.
Medical subject headings
- Optogenetics
- Protein Engineering
- Recombinant Proteins