Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28298421.
- Also identified by DOI 10.1126/scitranslmed.aai7521 and PMC identifier 5562351.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A goal for an HIV-1 vaccine is to overcome virus variability by inducing broadly neutralizing antibodies (bnAbs). One key target of bnAbs is the glycan-polypeptide at the base of the envelope (Env) third variable loop (V3). We have designed and synthesized a homogeneous minimal immunogen with high-mannose glycans reflective of a native Env V3-glycan bnAb epitope (Man<sub>9</sub>-V3). V3-glycan bnAbs bound to Man<sub>9</sub>-V3 glycopeptide and native-like gp140 trimers with similar affinities. Fluorophore-labeled Man<sub>9</sub>-V3 glycopeptides bound to bnAb memory B cells and were able to be used to isolate a V3-glycan bnAb from an HIV-1-infected individual. In rhesus macaques, immunization with Man<sub>9</sub>-V3 induced V3-glycan-targeted antibodies. Thus, the Man<sub>9</sub>-V3 glycopeptide closely mimics an HIV-1 V3-glycan bnAb epitope and can be used to isolate V3-glycan bnAbs.
Medical subject headings
- Antibodies, Neutralizing
- Epitopes
- Glycopeptides
- HIV-1
- Molecular Mimicry