Light-sensing via hydrogen peroxide and a peroxiredoxin.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28337980.
- Also identified by DOI 10.1038/ncomms14791 and PMC identifier 5376668.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) signal that is sensed by the peroxiredoxin Tsa1 and transduced to thioredoxin, to counteract PKA-dependent Msn2 phosphorylation. Upon H<sub>2</sub>O<sub>2</sub>, the nuclear retention of PKA catalytic subunits, which contributes to delayed Msn2 nuclear concentration, is antagonized in a Tsa1-dependent manner. Conversely, peroxiredoxin hyperoxidation interrupts the H<sub>2</sub>O<sub>2</sub> signal and drives Msn2 oscillations by superimposing on PKA feedback regulation. Our data identify a mechanism by which light could be sensed in all cells lacking dedicated photoreceptors. In particular, the use of H<sub>2</sub>O<sub>2</sub> as a second messenger in signalling is common to Msn2 oscillations and to light-induced entrainment of circadian rhythms and suggests conserved roles for peroxiredoxins in endogenous rhythms.
Medical subject headings
- Hydrogen Peroxide
- Light Signal Transduction
- Peroxidases
- Peroxiredoxins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins