Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28346401.
- Also identified by DOI 10.1038/nbt.3833.
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Abstract
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
Medical subject headings
- CRISPR-Associated Proteins
- DNA, Plant
- Gene Editing
- Solanum lycopersicum
- Mutagenesis, Site-Directed
- Oryza