Nasosorption as a Minimally Invasive Sampling Procedure: Mucosal Viral Load and Inflammation in Primary RSV Bronchiolitis.
prospective_cohort · Level II
Where this comes from
- Record sourced from PubMed, PMID 28368490.
- Also identified by DOI 10.1093/infdis/jix150 and PMC identifier 5441107.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting. Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)-positive, 12 were RSV-negative. Infants were sampled by nasosorption and nasopharyngeal aspiration (NPA). Nasosorption was well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not NPA) correlated with length of hospital stay (P = .04) and requirement for mechanical ventilation (P = .03). Nasosorption (but not NPA) levels of interferon γ, interleukin 1β, CCL5/RANTES, and interleukin 10 (IL-10) were elevated in RSV+ bronchiolitis (all P < .05), furthermore CCL5 and IL-10 correlated with RSV load (P < .05). Nasosorption allowed measurement of RSV load and the mucosal inflammatory response in infants.
Medical subject headings
- Bronchiolitis, Viral
- Inflammation
- Nasal Mucosa
- Respiratory Syncytial Virus Infections
- Viral Load