Bioacoustic-enabled patterning of human iPSC-derived cardiomyocytes into 3D cardiac tissue.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28376365.
- Also identified by DOI 10.1016/j.biomaterials.2017.03.037 and PMC identifier 5446052.
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Abstract
The creation of physiologically-relevant human cardiac tissue with defined cell structure and function is essential for a wide variety of therapeutic, diagnostic, and drug screening applications. Here we report a new scalable method using Faraday waves to enable rapid aggregation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) into predefined 3D constructs. At packing densities that approximate native myocardium (10<sup>8</sup>-10<sup>9</sup> cells/ml), these hiPSC-CM-derived 3D tissues demonstrate significantly improved cell viability, metabolic activity, and intercellular connection when compared to constructs with random cell distribution. Moreover, the patterned hiPSC-CMs within the constructs exhibit significantly greater levels of contractile stress, beat frequency, and contraction-relaxation rates, suggesting their improved maturation. Our results demonstrate a novel application of Faraday waves to create stem cell-derived 3D cardiac tissue that resembles the cellular architecture of a native heart tissue for diverse basic research and clinical applications.
Medical subject headings
- Bioprinting
- Induced Pluripotent Stem Cells
- Myocytes, Cardiac
- Tissue Engineering