DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 28394337.
- Also identified by DOI 10.1038/nmeth.4257 and PMC identifier 5419720.
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Abstract
The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.
Medical subject headings
- ADP Ribose Transferases
- Actin Cytoskeleton
- Actins
- Gelsolin
- Peptides
- Recombinant Fusion Proteins
- Virulence Factors