Improving specificity of Bordetella pertussis detection using a four target real-time PCR.
retrospective_cohort · Level III
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- Record sourced from PubMed, PMID 28403204.
- Also identified by DOI 10.1371/journal.pone.0175587 and PMC identifier 5389834.
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Abstract
The incidence of whooping cough, a contagious respiratory disease caused by Bordetella pertussis, is on the rise despite existing vaccination programmes. Similar, though usually milder, respiratory symptoms may be caused by other members of the Bordetella genus: B. parapertussis, B. holmesii, and B. bronchiseptica. Pertussis diagnosis is mostly done using PCR, but the use of multiple targets is necessary in order to differentiate the different Bordetella spp. with sufficient sensitivity and specificity. In this study we evaluate a multiplex PCR assay for the differentiation of B. pertussis from other Bordetella spp., using the targets IS481, IS1001, IS1002, and recA. Moreover, we retrospectively explore the epidemiology of Bordetella spp. infections in Belgium, using the aforementioned assay over a three-year period, from 2013 until 2015.
Medical subject headings
- Bordetella pertussis
- Whooping Cough