QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28406948.
- Also identified by DOI 10.1371/journal.pone.0175146 and PMC identifier 5390991.
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Abstract
Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and short overlapping ends. Our method called QuickLib should find many applications in synthetic biology; as an example, we easily prepared genetic libraries of Escherichia coli expressing billions of different backbone cyclic peptides.
Medical subject headings
- Cloning, Molecular
- Escherichia coli
- Oligodeoxyribonucleotides
- Peptide Library
- Plasmids
- Polymerase Chain Reaction