Normal vibrational modes of phospholipid bilayers observed by low-frequency Raman scattering.

Abstract

Low-frequency Raman spectra of multilamellar vesicles made either of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) have been studied in a wide temperature range. Below 0^{∘}C two peaks are found at frequencies around 8-9 and 14-17cm^{-1} and attributed to the normal vibrational modes of the phospholipid bilayer, which are determined by the bilayer thickness and stiffness (elastic modulus). The spectral positions of the peaks depend on the temperature and the bilayer composition. It is suggested that the ratio of the intensities of the first and second peaks can serve as a measure of the interleaflet elastic coupling. The addition of cholesterol to the phospholipid bilayer leads to peak shift and broadening, which may be assigned to the composition heterogeneities commonly attributed to the lipid raft formation.