SCnorm: robust normalization of single-cell RNA-seq data.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 28418000.
- Also identified by DOI 10.1038/nmeth.4263 and PMC identifier 5473255.
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Abstract
The normalization of RNA-seq data is essential for accurate downstream inference, but the assumptions upon which most normalization methods are based are not applicable in the single-cell setting. Consequently, applying existing normalization methods to single-cell RNA-seq data introduces artifacts that bias downstream analyses. To address this, we introduce SCnorm for accurate and efficient normalization of single-cell RNA-seq data.
Medical subject headings
- Algorithms
- High-Throughput Nucleotide Sequencing
- RNA
- Sequence Analysis, RNA
- Single-Cell Analysis
- Transcriptome