Transparent and Gas-Permeable Liquid Marbles for Culturing and Drug Sensitivity Test of Tumor Spheroids.

Li, Hualin; Liu, Peng; Kaur, Guneet; Yao, Xi; Yang, Mengsu · Adv Healthc Mater · 2017

basic_science · Level V

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Abstract

Transparent nanoparticle-based liquid marbles with high gas-permeability are prepared to culture tumor spheroids in 3D without the need of supplementary growth factor. The culturing process of spheroids from a population of cancer cells or a single cell in the transparent liquid marbles can be optically recorded continuously. Compared to monolayer cells and spheroids generated in multiwell plate, tumor spheroids cultured in the liquid marbles show enhanced viability under the treatment of chemotherapeutic drugs and small interfering RNA.

Medical subject headings