Chainy: an universal tool for standardized relative quantification in real-time PCR.
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- Record sourced from PubMed, PMID 28453678.
- Also identified by DOI 10.1093/bioinformatics/btw839.
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Abstract
Chainy is a cross-platform web tool providing systematic pipelines and steady criteria to process real-time PCR data, including the calculation of efficiencies from raw data by kinetic methods, evaluation of the suitability of multiple references, standardized normalization using one or more references, and group-wise relative quantification statistical testing. We illustrate the utility of Chainy for differential expression and chromatin immunoprecipitation enrichment (ChIP-QPCR) analysis. Chainy is open source and freely available at http://maplab.cat/chainy. imallona@igtp.cat. Supplementary data are available at Bioinformatics online.
Medical subject headings
- Chromatin Immunoprecipitation
- Real-Time Polymerase Chain Reaction
- Software